Coding

Part:BBa_K3782008:Design

Designed by: Cyntia Lukala   Group: iGEM21_UNILausanne   (2021-10-17)


Pseudomonas syringae DSM50252 derived tailocin gene cluster


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2030
    Illegal EcoRI site found at 5264
    Illegal EcoRI site found at 11011
    Illegal PstI site found at 182
    Illegal PstI site found at 606
    Illegal PstI site found at 2085
    Illegal PstI site found at 2348
    Illegal PstI site found at 2387
    Illegal PstI site found at 3804
    Illegal PstI site found at 5597
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2030
    Illegal EcoRI site found at 5264
    Illegal EcoRI site found at 11011
    Illegal PstI site found at 182
    Illegal PstI site found at 606
    Illegal PstI site found at 2085
    Illegal PstI site found at 2348
    Illegal PstI site found at 2387
    Illegal PstI site found at 3804
    Illegal PstI site found at 5597
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2030
    Illegal EcoRI site found at 5264
    Illegal EcoRI site found at 11011
    Illegal BglII site found at 11166
    Illegal BamHI site found at 1330
    Illegal BamHI site found at 10503
    Illegal XhoI site found at 9613
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2030
    Illegal EcoRI site found at 5264
    Illegal EcoRI site found at 11011
    Illegal PstI site found at 182
    Illegal PstI site found at 606
    Illegal PstI site found at 2085
    Illegal PstI site found at 2348
    Illegal PstI site found at 2387
    Illegal PstI site found at 3804
    Illegal PstI site found at 5597
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2030
    Illegal EcoRI site found at 5264
    Illegal EcoRI site found at 11011
    Illegal PstI site found at 182
    Illegal PstI site found at 606
    Illegal PstI site found at 2085
    Illegal PstI site found at 2348
    Illegal PstI site found at 2387
    Illegal PstI site found at 3804
    Illegal PstI site found at 5597
    Illegal NgoMIV site found at 1365
    Illegal NgoMIV site found at 1790
    Illegal NgoMIV site found at 2072
    Illegal NgoMIV site found at 2485
    Illegal NgoMIV site found at 2747
    Illegal NgoMIV site found at 3574
    Illegal NgoMIV site found at 4028
    Illegal AgeI site found at 1032
    Illegal AgeI site found at 2377
    Illegal AgeI site found at 3245
    Illegal AgeI site found at 5176
    Illegal AgeI site found at 6840
    Illegal AgeI site found at 8051
    Illegal AgeI site found at 9867
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7258
    Illegal BsaI site found at 8089
    Illegal BsaI site found at 11930
    Illegal BsaI site found at 12494
    Illegal BsaI.rc site found at 1134
    Illegal BsaI.rc site found at 3436
    Illegal BsaI.rc site found at 3905
    Illegal BsaI.rc site found at 4016
    Illegal BsaI.rc site found at 7805
    Illegal SapI.rc site found at 6651


Design Notes

The sequence was not changed from its original.


Source

The source of this tailocin gene cluster is Pseudomonas syringae DSM50252. Its sequence was retrieved from GenBank and phaster.ca. https://phaster.ca/submissions/AEAN00000000.1 https://www.ncbi.nlm.nih.gov/nuccore/AEAN00000000.1

References